We've built a small tool for planning proteolytic digests. You enter a UniProt accession or paste a sequence, choose one or two enzymes, and the page shows the cleavage sites, peptide masses and m/z values, along with how much of the sequence falls inside your measurement range.
- We've included 13 enzyme presets, each with a reference to the primary literature, or you can define your own specificity across P4–P4′, including positions defined by exclusion ("not Pro")
- Two enzymes at a time, with cut sites coloured per enzyme
- Monoisotopic and average masses, and m/z for the selected charge states
- Fixed cysteine modification: carbamidomethyl (IAA, +57), methylthio (MMTS, +46) or none
- Sequence coverage for the chosen measurement range, updated as you type
- Hovering a peptide shows its position in the protein, its sequence and its m/z at each charge
- Settings are kept in the URL, so a permalink reopens the same digest
- It runs in the browser; the only request it makes is for an accession you fetch from UniProt
Needs no installation or account. → https://mslab-ibb.pl/digest