The Laboratory routinely performs qualitative analysis of protein samples of various complexity - from pure proteins to complex mixtures.
The standard procedure for qualitative MS analysis of a protein sample includes following steps:
- reduction and alkylation of protein disulfide bonds, followed by tryptic digestion to obtain peptide mixture;
- liquid chromatography (LC) separation of a sample, MS measurement of peptides and their fragmentation spectra (tandem mass spectrometry);
- searching of acquired spectra against; a protein sequence database of choice (NCBI, UniProt or customer-supplied database) using Mascot search engine, Proteome Discoverer, MaxQuant, DIA-NN or Spectronaut.
The types of samples we routinely perform qualitative analysis on are:
- gel bands - stained either with Coomassie or silver,
- pellets,
- protein solutions,
- IP beads.
Most often the preparation protocol needs to be consulted with our staff in advance in order to ensure compatibility. Please, don't hesitate to contact us.
Information on the status of analysis and details concerning data access will be sent to the email address provided in the request form.
Standard qualitative analysis is performed as a service and can be ordered online through our order processing system.